| Autor: |
Hitoshi Endo, Keiya Ozawa, Mie Uchida, Tomoyuki Endoh, Yoshifumi Kashii, Norio Komatsu, Masaru Tanaka, Kenichi Sawada, Keita Kirito, Tomoko Watanabe |
| Rok vydání: |
2001 |
| Předmět: |
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| Zdroj: |
Journal of Biological Chemistry. 276:15082-15089 |
| ISSN: |
0021-9258 |
| DOI: |
10.1074/jbc.m007958200 |
| Popis: |
FKHRL1, a member of the Forkhead transcription factor family, is one of the downstream molecules of phosphatidylinositol 3-kinase-Akt. This molecule is a mammalian homolog of DAF-16, which plays an important role in the longevity ofCaenorhabditis elegans. In this study we found that Akt and FKHRL1 proteins were detectable in highly purified normal human megakaryocytes and that these molecules were actually phosphorylated by thrombopoietin (TPO). To clarify the functional role of FKHRL1 in TPO signaling, we established a tetracycline-inducible system in the human TPO-dependent leukemia cell line UT-7/TPO. Induced expression of active FKHRL1 led to cell cycle arrest at G0/G1 phase in this cell line. These results suggest that FKHRL1 plays an important role in the cell cycle of megakaryocytic cells as one of the downstream target molecules of phosphatidylinositol 3-kinase-Akt, presumably mediated through the activation or inactivation of cell cycle-associated gene(s). |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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