Expression ofRSOsPR10in rice roots is antagonistically regulated by jasmonate/ethylene and salicylic acid via the activator OsERF87 and the repressor OsWRKY76, respectively
| Autor: | Yoko Nishizawa, Tomokazu Koshiba, Atsuko Gyohda, Makiko Tominaga, Hiromi Suzuki, Takashi Okamoto, Kazunari Nakajima, Naoki Yokotani, Takahiro Yamamoto, Hisakazu Yamane, Takeshi Nishimura, Eiichi Minami, Koji Miyamoto, Yuri Yoshida, Kazunori Okada |
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| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Ecology Activator (genetics) Jasmonic acid Lateral root food and beverages Repressor Promoter Plant Science 01 natural sciences Biochemistry Genetics and Molecular Biology (miscellaneous) Genetically modified rice Cell biology 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology chemistry Jasmonate Transcription factor Ecology Evolution Behavior and Systematics 010606 plant biology & botany |
| Zdroj: | Plant Direct. 2:e00049 |
| ISSN: | 2475-4455 |
| DOI: | 10.1002/pld3.49 |
| Popis: | Plant roots play important roles in absorbing water and nutrients, and in tolerance against environmental stresses. Previously, we identified a rice root-specific pathogenesis-related protein (RSOsPR10) induced by drought, salt, and wounding. RSOsPR10 expression is strongly induced by jasmonate (JA)/ethylene (ET), but suppressed by salicylic acid (SA). Here, we analyzed the promoter activity of RSOsPR10. Analyses of transgenic rice lines harboring different-length promoter::β-glucuronidase (GUS) constructs showed that the 3-kb promoter region is indispensable for JA/ET induction, SA repression, and root-specific expression. In the JA-treated 3K-promoter::GUS line, GUS activity was mainly observed at lateral root primordia. Transient expression in roots using a dual luciferase (LUC) assay with different-length promoter::LUC constructs demonstrated that the novel transcription factor OsERF87 induced 3K-promoter::LUC expression through binding to GCC-cis elements. In contrast, the SA-inducible OsWRKY76 transcription factor strongly repressed the JA-inducible and OsERF87-dependent expression of RSOsPR10. RSOsPR10 was expressed at lower levels in OsWRKY76-overexpressing rice, but at higher levels in OsWRKY76-knockout rice, compared with wild type. These results show that two transcription factors, OsERF87 and OsWRKY76, antagonistically regulate RSOsPR10 expression through binding to the same promoter. This mechanism represents a fine-tuning system to sense the balance between JA/ET and SA signaling in plants under environmental stress. |
| Databáze: | OpenAIRE |
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