Simultaneous Analysis of Free/Bound Phytosterols and Diosgenin in Taiwanese Yams
Autor: | Huei-Ju Wang, Ting Jang Lu, Yu-Chih Lin, Chia-Wen Yu |
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Rok vydání: | 2016 |
Předmět: |
0301 basic medicine
Chromatography Stigmasterol biology Campesterol Phytosterol 010401 analytical chemistry Diosgenin biology.organism_classification 01 natural sciences Applied Microbiology and Biotechnology High-performance liquid chromatography 0104 chemical sciences Analytical Chemistry 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology chemistry Chromatography detector Dioscorea Gas chromatography Safety Risk Reliability and Quality Safety Research Food Science |
Zdroj: | Food Analytical Methods. 10:1172-1180 |
ISSN: | 1936-976X 1936-9751 |
Popis: | Gas chromatography (GC) and high performance liquid chromatography (HPLC) methods were developed for the simultaneous analysis of phytosterols and diosgenin in two representative Taiwanese yams, Dioscorea pseudojaponica Yamamoto and Dioscorea alata L. The major phytosterols found were β-sitosterol, stigmasterol, and campesterol. GC afforded good separation of phytosterols and diosgenin, with the diosgenin peak appearing between stigmasterol and β-sitosterol. For HPLC, UV detection had higher sensitivity (detection limits 0.03–0.09 μg); however, evaporative light scattering detection (ELSD) achieved higher noise tolerance and did not show baseline interference from solvent gradients. In addition, mixed solvents for extraction before acid hydrolysis, which is necessary to avoid fibers and acid hydrolysates binding to the target compounds, were evaluated. Phytosterol contents of 30.1 and 9.9 mg/100 g were found in D. pseudojaponica Yamamoto and D. alata L., respectively. Diosgenin was only found in D. pseudojaponica Yamamoto at a content of 15.2 mg/100 g. |
Databáze: | OpenAIRE |
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