Inhibition of mould growth by oligomycin during quantitative analysis of yeasts

Autor: Hansjörg Prillinger, Frieda Eliskases-Lechner
Rok vydání: 1996
Předmět:
Zdroj: Journal of Dairy Research. 63:483-488
ISSN: 1469-7629
0022-0299
Popis: Quantitative analysis of yeasts can be difficult in mould-contaminated samples. Particularly in the presence of fast growing moulds, counting yeasts may be difficult or even impossible. Furthermore, in foodstuffs made with mould cultures, mould counts usually exceed yeast counts. Isolation of yeasts in mould-contaminated samples is often effected by the incorporation of sodium or calcium propionate in the media (Bowen & Beech, 1967 ; Rale & Vakil, 1984). Another possible method of inhibiting moulds, less effective than the use of propionate, is to use a medium with the low pH of 3.5 (Devoyod & Sponem, 1970). In addition, mould inhibition through the use of antibiotics is a possibility. Sheridan (1992) reported the inhibition of moulds by oligomycin, but no detailed information was given on the required concentrations or the effect on the growth of yeasts. The aim of this study was therefore to examine the inhibiting effect of oligomycin on different mould species, especially on commercial cultures used in cheesemaking. and to determine the concentrations that do not influence the growth of the yeast species commonly found in milk products.
Databáze: OpenAIRE