Biocatalysis in Organic Solvents with a Polymer-Bound Horseradish Peroxidase
| Autor: | Martine Urrutigoïty, Jerome Souppe |
|---|---|
| Rok vydání: | 1989 |
| Předmět: |
chemistry.chemical_classification
Chloroform biology technology industry and agriculture Periodate Polymer Biochemistry Horseradish peroxidase Toluene Catalysis chemistry.chemical_compound Enzyme chemistry Biocatalysis biology.protein Organic chemistry Moiety General Agricultural and Biological Sciences Biotechnology |
| Zdroj: | Biocatalysis. 2:145-149 |
| ISSN: | 0886-4454 |
| DOI: | 10.3109/10242428909003655 |
| Popis: | This paper describes the preparation of polyethyleneglycol-bound horseradish peroxidase. Coupling with the polymer occurs via the glycolic moiety of the protein after an optimised oxidation process with periodate. Analysis of the modified enzyme shows that three chains of polymer are attached to the protein, which then becomes soluble and active in both chloroform and toluene. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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