Multiplex PCR for genes encoding prevalent OXA and NDM-1 carbapenemases in Acinetobacter
| Autor: | R. Soniya, Abhisek Routray, Radha Madhavan, P. Lavanya |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Imipenem
biology Antimicrobial susceptibility biochemical phenomena metabolism and nutrition Acinetobacter biology.organism_classification Microbiology Minimum inhibitory concentration Multiplex polymerase chain reaction polycyclic compounds medicine bacteria Carbapenem resistance genes Carbapenem resistant Acinetobacter Gene medicine.drug |
| Zdroj: | Journal of Pharmacy Research. 7:324-326 |
| ISSN: | 0974-6943 |
| DOI: | 10.1016/j.jopr.2013.03.026 |
| Popis: | Aims Carbapenem resistant Acinetobacter are being increasingly reported worldwide. We describe a novel multiplex PCR assay for simultaneous detection of carbapenem resistance genes in clinical isolates of multi drug-resistant Acinetobacter . Materials and methods Strains of Acinetobacter collected from different clinical samples were subjected antimicrobial susceptibility testing. Strains which were found showing resistance to imipenem by both disk diffusion and minimum inhibitory concentration (MIC) were analysed for presence of oxacillinase genes i.e. OXA-23, OXA-58 (class D) and metallo-β-lactamase gene i.e. New Delhi metallo-β-lactamase i.e. NDM-1 (class B) by using multiplex PCR. Results Among 175 strains of Acinetobacter collected from clinical samples, 45 (25.71%) strains showed imipenem resistance both by disk diffusion and MIC, among which 14 (31%) were positive for NDM-1 gene, 19 (42.2%) were positive for OXA-58 gene and all strains 45 (100%) were positive for OXA-23 gene. Conclusion This study emphasizes the dissemination of OXA cabapenemase genes and its co-existence with NDM-1 genes in carbapenem resistant Acinetobacte r isolates. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|