Internal standardization of LA-ICP-MS immuno imaging via printing of universal metal spiked inks onto tissue sections
| Autor: | Boris Neumann, Hartmut Schlüter, Michael W. Linscheid, Ronald Simon, Guido Sauter, Sandra Techritz, Franz Theuring, Norbert Jakubowski, Larissa Mueller, Simone Hoesl |
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| Rok vydání: | 2016 |
| Předmět: |
Reproducibility
Materials science Standardization 010401 analytical chemistry Analytical chemistry 02 engineering and technology 021001 nanoscience & nanotechnology Mass spectrometry 01 natural sciences Paraffin embedded 0104 chemical sciences Analytical Chemistry Tissue sections La icp ms Constant density Multiplex 0210 nano-technology Spectroscopy Biomedical engineering |
| Zdroj: | Journal of Analytical Atomic Spectrometry. 31:801-808 |
| ISSN: | 1364-5544 0267-9477 |
| DOI: | 10.1039/c5ja00409h |
| Popis: | Formalin-fixed paraffin-embedded (FFPE) specimen from biopsy materials are a widespread sample format for pathologists and medical researchers. Pathologists are archiving vast numbers of FFPE samples which can be stored for decades. Conventional immunohistochemical staining (IHC) of biomarkers on FFPE tissue sections is one of the most important analytical techniques for cancer diagnosis and pathology in general. However standardization for IHC samples and quality management is tedious and differs significantly from clinic to clinic. Combining established IHC staining strategies with modern mass spectrometry mediated methods would increase it's potential and enable access of large FFPE archives for multiplexed quantitation purposes. In this work element mass spectrometry and a new ink-jet printed internal standardization approach was successfully combined with IHC staining to facilitate quantitative multiplex assays for archived FFPE samples. The printing strategy improves elemental image resolution and reproducibility of paraffin embedded breast cancer tissue sections in laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) using conventional IHC staining as a model system to investigate the new capabilities of this technique. For the internal standardization we applied a conventional CD-ink-jet printer to print a metal spiked ink onto the top of thin layer tissue sections with constant density. Printing was carried out in a direct comparison to an iodination of the tissue section as previously described as an alternative standardization method. The use of the printed internal standard allowed correction of the fluctuation during the laser ablation process and compensated instrumental drift effects. Mediated by the ink correction approach we achieved better signal-to-background-ratios (SBR) of 74 and better spatial resolution of 30 μm compared to iodination (SBR = 23). This improved performance was demonstrated on tumorous areas in FFPE breast cancer tissue sections and allowing detection of Her-2 in tumorous areas of this tissue with significantly improved contrast. |
| Databáze: | OpenAIRE |
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