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Publisher Summary This chapter describes the α-helical form of M13 major coat protein and its properties in relation to the in vivo biological processes. M13 bacteriophage and the closely related phages, fl and fd, are Escherichia coli -specific filamentous phages belonging to the genus, Inovirus. The chapter discusses the molecular properties of the membrane-bound bacteriophage disassembly and assembly processes by studying the structural and functional behavior of the major coat protein of bacteriophage M13 and related phages, when incorporated into model membrane systems. Various spectroscopic techniques have been applied that provide detailed information about these reconstituted systems, whereas biochemical separation techniques allow purifying and characterizing these systems. The chapter discusses the role of M13 coat protein in the infection process, leading to conditions to reconstitute the protein in its native state into model membrane systems. In addition, the structure of the coat protein and protein–lipid interactions is discussed in the chapter. |
