Treating Titanium Particle-Induced Inflammation with Genetically Modified NF-κB Sensing IL-4 Secreting or Preconditioned Mesenchymal Stem Cells in Vitro
| Autor: | Monica Romero-Lopez, Jukka Pajarinen, Masaya Ueno, Masahiro Maruyama, Yusuke Kohno, Zhenyu Yao, Chi-Wen Lo, Stuart B. Goodman, Karthik Nathan, Eemeli Jämsen, Tzuhua Lin |
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| Rok vydání: | 2019 |
| Předmět: |
Stromal cell
Osteolysis Chemistry 0206 medical engineering Mesenchymal stem cell Biomedical Engineering Periprosthetic Inflammation 02 engineering and technology 021001 nanoscience & nanotechnology medicine.disease 020601 biomedical engineering Biomaterials medicine.anatomical_structure medicine Cancer research Macrophage Bone marrow medicine.symptom 0210 nano-technology Interleukin 4 |
| Zdroj: | ACS Biomaterials Science & Engineering. 5:3032-3038 |
| ISSN: | 2373-9878 |
| Popis: | Titanium and titanium-based alloys are widely used in orthopaedic implants. Total joint replacement is very successful; however, the foreign body response and chronic inflammation caused by implant-derived biomaterial debris still remain as unsolved issues. Aseptic loosening accompanied by wear debris-induced osteolysis (bone loss) is one of the most frequent causes for late failure and revision surgery. Mesenchymal stem cells (MSCs) and IL-4 may be possible treatment strategies because of their immunomodulatory properties. We investigated the efficacy of novel MSC-based treatments on immunomodulation and osteogenic differentiation in an innovative cell coculture model of titanium particle-induced inflammation in the periprosthetic tissues. MSCs and macrophages were collected from the bone marrow of Balb/c mice. Both MSCs and macrophages (representing endogenous cells at the periprosthetic tissue) were seeded on the bottom wells of the 24-well transwell plates. We generated genetically modified NF-κB sens... |
| Databáze: | OpenAIRE |
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