The Influence of Cytokines on the Adhesion of Renal Cancer Cells to Endothelium
| Autor: | Jeannine Alexander, Eric A. Klein, Frank Steinbach, Kazunari Tanabe, Mark Edinger, Walburgis Brenner, Andrew C. Novick, Raymond R. Tubbs, Michael Stockle |
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| Rok vydání: | 1996 |
| Předmět: |
Pathology
medicine.medical_specialty Endothelium biology business.industry Cell adhesion molecule Urology Soluble cell adhesion molecules Adhesion Intercellular adhesion molecule Umbilical vein medicine.anatomical_structure E-selectin cardiovascular system Cancer research biology.protein Medicine business Cell adhesion |
| Zdroj: | Journal of Urology. 155:743-748 |
| ISSN: | 1527-3792 0022-5347 |
| DOI: | 10.1016/s0022-5347(01)66513-3 |
| Popis: | Purpose: The development of tumor metastasis requires direct adhesive interactions between tumor cells and vascular endothelium. We examined the adherence of renal cell carcinoma (RCC) lines to endothelium after stimulation with different cytokines that include expression of the vascular adhesion molecules endothelial leukocyte adhesion molecule (ELAM)-1, vascular cell adhesion molecule (VCAM)-1 and intercellular adhesion molecule (ICAM)-1.Materials and Methods: Human umbilical vein endothelial cells (HUVEC) were used to determine the adhesion of the RCC lines CCF-RC1, 2 and 7 to endothelium. Expression of cell adhesion molecules (CAM) on HUVEC and RCC lines was measured with immunoflowcytometry.Results: Stimulation of HUVEC with rIl-1 beta, rTNF-alpha, or PMA resulted in a time-dependent 1.4- to 2.9-fold increase of RCC adhesion to HUVEC. Significant increased tumor cell binding was observed after 4 hours and paralleled the time-dependent induction of ELAM-1 and VCAM-1. Immunocytometry demonstrat... |
| Databáze: | OpenAIRE |
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