Inverse correlation between the number of CXCR3+macrophages and the severity of inflammatory lesions in Sjögren's syndrome salivary glands: A pilot study
| Autor: | Koichi Kani, Naozumi Ishimaru, Masayuki Azuma, Tomoko Yamanoi, Koh-ichi Nakashiro, Keiko Aota |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Cancer Research Chemokine Pathology medicine.medical_specialty Ductal cells CXCR3 Pathology and Forensic Medicine 03 medical and health sciences 0302 clinical medicine Immune system stomatognathic system immune system diseases Medicine CXCL10 skin and connective tissue diseases Innate immune system biology business.industry hemic and immune systems stomatognathic diseases 030104 developmental biology Otorhinolaryngology biology.protein Periodontics Oral Surgery Antibody business CD163 030215 immunology |
| Zdroj: | Journal of Oral Pathology & Medicine. 47:710-718 |
| ISSN: | 0904-2512 |
| Popis: | Background: Mechanisms underlying immune cells' recruitment and activation into the inflammatory lesions of lip salivary glands (LSGs) from primary Sjogren's syndrome (pSS) patients are incompletely understood. Chemokines play pivotal roles in these processes, so we investigated the clinical significance of chemokine receptor CXCR3 and its ligands in the autoimmune lesions of pSS. Methods: We histologically determined the grade of LSG samples from 22 pSS patients and subjected the samples to immunofluorescence analysis to determine the expressions of CXCR3 and its ligands: CXCL9, CXCL10, and CXCL11. To identify the immune cells expressing CXCR3 in the LSGs, we performed double immunofluorescence analysis using antibodies against CD3 (pan-T cells), CD80 (M1 macrophages), CD163 (M2 macrophage), and CD123 (plasmacytoid dendritic cells: pDCs). The relationship between the grade of lymphocytic infiltration and the number of positively stained cells was analyzed by Spearman's rank correlation test. Results: The expressions of CXCL9 and CXCL10 showed particularly intense staining in the LSG samples' ductal cells. The CXCR3 expression was detected mainly in CD80+ and CD163+ macrophages. The number of CXCR3+CD163+ macrophages inversely correlated with the LSG inflammatory lesions' severity (rs= −0.777, p |
| Databáze: | OpenAIRE |
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