| Autor: |
Amalio Telenti, Bernard Hirschel, Andri Rauch, Thierry Buclin, Laurent A. Decosterd, Alexandra Calmy, Matthias Cavassini, Manel Aouri |
| Rok vydání: |
2013 |
| Předmět: |
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| Zdroj: |
Journal of Mass Spectrometry. 48:616-625 |
| ISSN: |
1076-5174 |
| DOI: |
10.1002/jms.3200 |
| Popis: |
Because of the large variability in the pharmacokinetics of anti-HIV drugs, therapeutic drug monitoring in patients may contribute to optimize the overall efficacy and safety of antiretroviral therapy. An LC–MS/MS method for the simultaneous assay in plasma of the novel antiretroviral agents rilpivirine (RPV) and elvitegravir (EVG) has been developed to that endeavor. Plasma samples (100 μL) extraction is performed by protein precipitation with acetonitrile, and the supernatant is subsequently diluted 1:1 with 20-mM ammonium acetate/MeOH 50:50. After reverse-phase chromatography, quantification of RPV and EVG, using matrix-matched calibration samples, is performed by electrospray ionization–triple quadrupole mass spectrometry by selected reaction monitoring detection using the positive mode. The stable isotopic-labeled compounds RPV-13C6 and EVG-D6 were used as internal standards. The method was validated according to FDA recommendations, including assessment of extraction yield, matrix effects variability ( |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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