| Autor: |
Ishimura Ken, Miyake Taijiro, Okano Keiichi, Maeta Hajime, Maeba Takashi, Moroguchi Akito |
| Rok vydání: |
2004 |
| Předmět: |
|
| Zdroj: |
Wound Repair and Regeneration. 12:A2-A2 |
| ISSN: |
1067-1927 |
| Popis: |
Aim: This study aimed to define the participation of local expression of IL-10 and TNFα around the anastomotic segment and clarify the effects of IL-10 and TNFα on anastomotic wound healing after digestive surgery under septic condition. Methods: Experimental animals were divided into LPS and control groups, which had either LPS or normal saline solution injected into the peritoneal cavity 24 h before transection and anastomosis of the colon. Immunohistochemical staining for IL-10 and TNFα on tissue samples were examined after the operation. Fibroblasts were cultured with IL-10 and TNFα, then proliferation rates were determined using the MTT assay. Type I collagen protein and MMP-I were detected by indirect immunofluorescence. Results: In the LPS group, IL-10 and TNFα expression were more enhanced than that in the control group 24 h after the operation. IL-10 reduced fibroblasts proliferation in dose dependent fasion in the presence of TNFα. Indirect immunofluorescence showed that IL-10 reduced type I collagen protein and increased MMP-I. Conclusions: Local expression of IL-10 and TNFα at the anastomotic site acted as an inhibitory factor in the wound healing process. IL-10 suppressed the remodeling of the extracellular matrix during wound healing. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
Plný text