Cytoplasmic staining of c-erbB-2 is not associated with the presence of detectable c-erbB-2 mRNA in breast cancer specimens
| Autor: | Angela Platt-Higgins, Philip S. Rudland, John H. R. Winstanley, Roger Barraclough, S Taylor |
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| Rok vydání: | 1998 |
| Předmět: |
Cancer Research
Pathology medicine.medical_specialty Messenger RNA animal structures biology Immunocytochemistry Mammary gland Molecular biology Receptor tyrosine kinase Staining Gene product medicine.anatomical_structure Oncology Cytoplasm Cancer cell medicine biology.protein skin and connective tissue diseases neoplasms |
| Zdroj: | International Journal of Cancer. 76:459-463 |
| ISSN: | 1097-0215 0020-7136 |
| DOI: | 10.1002/(sici)1097-0215(19980518)76:4<459::aid-ijc2>3.0.co;2-q |
| Popis: | The cell-surface receptor tyrosine kinase protein c-erbB-2 is immunocytochemically detected as membrane staining on the surface of cancer cells in 20-30% of cases of breast cancer, and its presence has been associated with poor prognosis for the patient. However, there have been numerous reports of immunocytochemical staining for c-erbB-2 solely in the cytoplasm of some normal and tumour specimens with frequently used anti-sera, and the presence of such staining has been difficult to interpret. It is not known for certain that cytoplasmic c-erbB-2 staining is an artefact of the immunocytochemical procedures used. Thus, mRNA for c-erbB-2 has been quantified in tumours exhibiting only cytoplasmic staining or varying levels of membrane staining using a sensitive, competitive PCR method. Whereas abundant levels of c-erbB-2 mRNA are found in tumours exhibiting membrane staining for c-erbB-2 and these levels correlate with the percentage of tumour cells showing membranous staining for c-erbB-2, the level of c-erbB-2 mRNA in tumours displaying only cytoplasmic staining is no higher than in c-erbB-2-negative specimens. |
| Databáze: | OpenAIRE |
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