Harzianin HB I, an 11-residue peptaibol from Trichoderma harzianum: isolation, sequence, solution synthesis and membrane activity

Autor:	Yann Prigent, Christophe Goulard, Catherine Auvin, Bernard Bodo, Isabelle Augeven-Bour, Sylvie Rebuffat
Rok vydání:	1997
Předmět:	chemistry.chemical_compound Residue (chemistry) Membrane Chromatography chemistry biology Peptaibol Proton NMR Membrane activity Trichoderma harzianum Tripeptide biology.organism_classification High-performance liquid chromatography
Zdroj:	Journal of the Chemical Society, Perkin Transactions 1. :1587-1594
ISSN:	1364-5463 0300-922X
DOI:	10.1039/a605629f
Popis:	Harzianin HB I is a minor component of the peptaibol mixture biosynthesized by a Trichoderma harzianum strain. It is isolated by a multi-step chromatography procedure, including HPLC; its sequence has been elucidated by LSIMS and two-dimensional 1H NMR experiments. This 11-residue peptaibol is an analogue of the 14-residue peptaibol harzianin HC IX, resulting from the deletion of the tripeptide Aib-Pro-Ala. The CD and NMR data (NOE data and amide proton temperature coefficients) are similar to those of HC IX, suggesting a right-handed helix-type conformation for the two peptides. Harzianin HB I was synthesized by the solution-phase method using BOP as coupling reagent. The membrane properties examined on liposomes are compared with those of other known peptaibols.
Databáze:	OpenAIRE
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