The Effect of 2-Hydroxypropyl-β-Cyclodextrin on Post-Thaw Parameters of Cryopreserved Jack and Stallion Semen

Autor:	Curtis R. Youngs, L.E. Evans, Rebecca J. Madison
Rok vydání:	2013
Předmět:	food.ingredient urogenital system Equine Acrosome reaction Ionophore Motility chemistry.chemical_element Semen Calcium Cryopreservation Andrology chemistry.chemical_compound food chemistry Yolk Ethylene glycol
Zdroj:	Journal of Equine Veterinary Science. 33:272-278
ISSN:	0737-0806
DOI:	10.1016/j.jevs.2012.07.021
Popis:	Cyclodextrins improve post-thaw viability and motility of semen as well as mediate cholesterol efflux and subsequent acrosome reaction in spermatozoa from several species. The objectives of this study were: (a) to assess the effect of prefreeze addition of 60 mM hydroxypropyl-β-cyclodextrin (β-CD) on post-thaw viability and motility of jack and stallion semen cryopreserved in ethylene glycol-based freezing extenders containing 5% or 20% (v/v) egg yolk (LEY and HEY, respectively), and (b) to evaluate the ability of 1 μM calcium ionophore A23187 and/or 60 mM β-CD to induce acrosome reaction in thawed jack and stallion spermatozoa. Post-thaw motility of spermatozoa cryopreserved in HEY was higher (P
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::cca8ea19b581bb0d6eef2b63faa05e7a https://doi.org/10.1016/j.jevs.2012.07.021 Zobrazit plný text záznamu Full Text from ScienceDirect