ENZYME IMMUNOASSAY KIT FOR THE DETERMINATION OF DEOXYNIVALENOL
| Autor: | G. S. Kornilovich, A. I. Shibeko, O. V. Sviridov, I. V. Gorbachova, L. N. Sukhenko, O. S. Kuprienko, A. A. Yastrebova, I. I. Vashkevich, T. V. Terentieva |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
chemistry.chemical_classification
Chromatography medicine.diagnostic_test 010405 organic chemistry Chemistry Organic Chemistry food and beverages 010402 general chemistry 01 natural sciences 0104 chemical sciences Analytical Chemistry Inorganic Chemistry Enzyme Chemistry (miscellaneous) Immunoassay medicine |
| Zdroj: | Proceedings of the National Academy of Sciences of Belarus, Chemical Series. 54:319-328 |
| ISSN: | 2524-2342 1561-8331 |
| DOI: | 10.29235/1561-8331-2018-54-3-319-328 |
| Popis: | A reagent kit EIA-DEOXYNIVALENOL for the determination of mycotoxin deoxynivalenol (DON) in feeds and foods by a direct competitive enzyme immunoassay using microtitration plate has been developed and tested. The basic components of the kit are polyclonal antibodies to DON, obtained as a result of immunization of rabbits with a conjugate of DON with bovine serum albumin and a conjugate of horseradish peroxidase with DON. The evaluated parameters of the kit and metrological characteristics of the technique of measurements correspond to the modern level of immunoassay development and provide the determination of DON content of agricultural products in a range of 0.2 to 6.0 mg/kg with proper accuracy and precision. The limit of quantitative determination of DON in grain and cereal foods does not exceed 0.2 mg/kg. |
| Databáze: | OpenAIRE |
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