Determination of aloesin in plasma by high-performance liquid chromatography as fluorescent 9-anthroyl derivative

Autor:	Jeong Hill Park, Kyeong Ho Kim, Jin Gyun Lee, Young G. Shin, Seung Ki Lee, Tae Hyung Cho, Sun Tack Oh
Rok vydání:	1998
Předmět:	Detection limit Chromatography Chemistry Organic Chemistry Extraction (chemistry) Reversed-phase chromatography High-performance liquid chromatography chemistry.chemical_compound Drug Discovery Molecular Medicine Methanol Derivatization Acetonitrile Quinuclidine
Zdroj:	Archives of Pharmacal Research. 21:651-656
ISSN:	1976-3786 0253-6269
DOI:	10.1007/bf02976752
Popis:	A sensitive high-performance liquid chromatographic (HPLC) method for the determination of aloesin in plasma was developed. After solid-phase extraction from plasma and derivatization of aloesin and compound AD-1, which was prepared from aloesin as a internal standard, with 9-anthroylnitrile in the presence of quinuclidine, the derivatives were separated on a Inertsil ODS-3 column using acetonitrile/methanol/water (3∶1∶6) as a mobile phase, and detected fluorimetrically at 460 nm with excitation at 360 nm. The detection limit of aloesin was 3.2 ng/ml in plasma (S/N=3).
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::cc8dfdc62a2bf241886923178d2d044e https://doi.org/10.1007/bf02976752 Zobrazit plný text záznamu Full text from SpringerLink