Addition of orange, pineapple and beet juices as extenders for cryopreservation of ram semen
| Autor: | Alexandre da Rocha Bozzi, Luiz Henrique Particelli, Carlos Henrique Cabral Viana, Célia Raquel Quirino, André Furugen Cesar de Andrade, Flávia Vieira de Freitas, Marina da Silva Passarelli, Eneiva Carla de Carvalho Celeghini, Héctor Javier Narvaez Bedoya, Alfonso Juventino Chay-Canul, Ricardo Lopes Dias da Costa |
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| Rok vydání: | 2023 |
| Předmět: | |
| Zdroj: | Ciência Animal Brasileira. 24 |
| ISSN: | 1809-6891 1518-2797 |
| DOI: | 10.1590/1809-6891v24e-72745e |
| Popis: | Searching for improvements in semen cryopreservation, natural substances are commonly studied focusing to improve the sperm quality. The aim of this study were evaluated the effect of adding orange, pineapple, and beet juices in different concentrations and combinations to the ram semen cryopreservation extender. Five ejaculates from five adult rams were used. The semen pool was diluted in egg yolk-based extender and mixed with the following 15 treatments (at a final concentration of 400.106 sptz/mL): orange 10% (O10) and 15% (O15); pineapple 10% (P10) and 15% (P15); beet 10% (B10) and 15% (B15); pineapple + orange 10% (PO10) and 15% (PO15); pineapple + beet 10% (PB10) and 15% (PB15); beet + orange 10% (BO10) and 15% (BO15); pineapple + beet + orange 10% (PBO10) and 15% (PBO15); and the control group (CON). Post-thaw in 0.25 mL straws semen quality analysis of cryopreserved semen was performed by CASA and flow cytometry. Analysis of variance (PROC GLM) was carried out and the averages were compared using the SNK test. Pearson's correlation test was also performed. No effect was noted in the addition of juices to the semen extender prior to cryopreservation. Post-thawed, although, statistically similar to the control group, the total motility of the B10 group reached acceptable standards of total motility. In addition, B10 group showed the highest values (p |
| Databáze: | OpenAIRE |
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