Quantitative genetic analysis of in vitro callus proliferation in alfalfa

Autor:	G. A. Kielly, S. R. Bowley
Rok vydání:	1997
Předmět:	education.field_of_study fungi Population food and beverages Plant Science Horticulture Heritability Biology Genetic analysis Diallel cross Callus Genetic variation Botany Cultivar Genetic variability education Agronomy and Crop Science
Zdroj:	Canadian Journal of Plant Science. 77:225-229
ISSN:	1918-1833 0008-4220
DOI:	10.4141/p96-001
Popis:	Genetic variability exists for in vitro callus growth within plant species, including alfalfa (Medicago sativa L.). The objective of this study was to investigate, using diallel crosses, the genetic variance components of in vitro callus production in tetraploid alfalfa. Nine genotypes were randomly chosen from among 60 plants from the cultivar Saranac and the experimental population OAC81-120R, which were previously characterized as being able to initiate callus. The group of nine plants was divided and crossed to generate two, six-plant diallel sets (A and B) with reciprocals; and the progeny were evaluated for callus production. Significant (P 2) for callus production on a plot mean basis were 73% and 61% for diallel A and B, respectively. Since the heritabilities were high, and no reciprocal effects were detected, breeding strategies employing recurrent selection should result in the development of high in vitro callus producing populations. Key words: Medicago sativa L., callus proliferation, tissue culture, quantitative genetics, heritability, alfalfa
Databáze:	OpenAIRE
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