Dual recombinantLactococcus lactisfor enhanced delivery of DNA vaccine reporter plasmid pPERDBY
| Autor: | Harish Padh, Drashya Sharma, Priti Desai, Bhrugu Yagnik |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
biology 030106 microbiology Immunology Lactococcus lactis RNA biology.organism_classification medicine.disease_cause Microbiology Molecular biology DNA vaccination law.invention 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology Plasmid chemistry Listeria monocytogenes law Virology Recombinant DNA medicine Internalin DNA |
| Zdroj: | Microbiology and Immunology. 61:123-129 |
| ISSN: | 0385-5600 |
| DOI: | 10.1111/1348-0421.12473 |
| Popis: | Food grade Lactococcus lactis has been widely used as an antigen and DNA delivery vehicle. We have previously reported the use of non-invasive L. lactis to deliver the newly constructed immunostimulatory DNA vaccine reporter plasmid, pPERDBY. In the present report, construction of dual recombinant L. lactis expressing internalin A of Listeria monocytogenes and harboring pPERDBY (LL InlA + pPERDBY) to enhance the efficiency of delivery of DNA by L. lactis is outlined. After confirmation and validation of LL InlA + pPERDBY, its DNA delivery potential was compared with previously developed non-invasive r- L. lactis::pPERDBY. The use of invasive L. lactis resulted in around threefold increases in the number of enhanced green fluorescent protein-expressing Caco-2 cells. These findings reinforce the prospective application of invasive strain of L. lactis for delivery of DNA/RNA and antigens. |
| Databáze: | OpenAIRE |
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