Abstract P540: Distinct Profile of Circulating Extracellular Vesicles in Normotensive Obese Humans

Autor: Uta Erdbruegger, Natalie Z Eichner, Nicole M Gilbertson, Christine Rudy, Luca Musante, Steven K Malin, Thu H Le
Rok vydání: 2017
Předmět:
Zdroj: Hypertension. 70
ISSN: 1524-4563
0194-911X
Popis: Hypertension (HTN) is a leading cause of cardiovascular disease. Early and non-invasive biomarkers of vascular dysfunction and end-organ damage in HTN are needed to optimize treatment for patients at risk for HTN. Extracellular vesicles (EVs) are potential candidate biomarkers that reflect occurrence of end-organ damage prior to symptom development. EVs are a heterogeneous population of submicron vesicles that shed from various cell types in the blood and carry markers from their parent cells. We hypothesized that obese adults with normotension have a distinct profile of circulating EVs. Twenty-two obese normotensive patients (Age: 62.5±8.9y, BMI 33.1±6.4 kg/m2) were enrolled in this cross-sectional study. After an overnight fast, systolic and diastolic blood pressure were 126±12.5mmHg and 68.7±8.2mmHg, respectively. Arterial stiffness was assessed by pulse wave velocity and augmentation index. Enumeration and phenotyping of platelet poor plasma EVs was performed using imaging flow cytometry. CD42 positive (platelet), CD31 (PECAM), CD105 (S-endoglin, endothelial), CD45 (leukocyte) positive and Annexin V (AnV) positive EVs were used as surface markers for circulating EVs. Annexin V (AnV) and S-Endoglin positive EVs (CD105+, AnV+) correlated significantly with the diastolic blood pressure (p=0.03, r=0.46). In addition, leukocyte derived EVs (CD45+, AnV+) correlated with pulse wave velocity (p=0.04, r=0.44). Other platelet derived EVs and AnV negative EVs did not correlate with blood pressure level or measures of vascular health. Subgroups of endothelial and leukocyte derived EVs correlate with diastolic blood pressure and arterial stiffness in obese normotensive adults. These findings suggest that the specific EV profile might reflect pathophysiologically significant pre-hypertensive blood pressure.
Databáze: OpenAIRE
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