Immunonephelometry of apolipoprotein A-II in plasma

Autor:	N Vinaimont, T A Musliner, Peter N. Herbert, Maryvonne Rosseneu, D Bernier, F Belpaire
Rok vydání:	1984
Předmět:	chemistry.chemical_compound Chromatography Triglyceride chemistry Coefficient of variation Biochemistry (medical) Clinical Biochemistry Blood plasma Apolipoprotein A-II Radioimmunoassay Sodium Cholate Nephelometry Dilution
Zdroj:	Clinical Chemistry. 30:234-237
ISSN:	1530-8561 0009-9147
DOI:	10.1093/clinchem/30.2.234
Popis:	A quantitative assay based on endpoint immunonephelometry was developed for human apolipoprotein A-II (apoA-II) in plasma or serum. Dilution of plasma samples with a 0.1 mol/L solution of sodium cholate enhanced the quantification. We used either purified apoA-II as the primary standard or plasma as a secondary standard. Results correlated well (r = 0.90) with those by a double-antibody radioimmunoassay for 63 serum samples from both normal and hyperlipemic individuals. The interassay coefficient of variation for the immunonephelometric assay was 7% within a working range between 0.05 and 0.7 microgram of apoA-II per sample (corresponding to a 1500-fold final dilution of serum). No extraction of samples with organic solvent is necessary if the triglyceride concentration is less than 4 g/L.
Databáze:	OpenAIRE
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