| Autor: | S. I. Malyshenko, S. D. Zvereva, L. G. Tyulkina, G. N. Raldugina |
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| Rok vydání: | 2003 |
| Předmět: | |
| Zdroj: | Russian Journal of Plant Physiology. 50:276-281 |
| ISSN: | 1021-4437 |
| DOI: | 10.1023/a:1022997803459 |
| Popis: | A protocol has been worked out for efficient regeneration and genetic transformation of summer rape (Brassica campestris L. var. oleifera). Cotyledons of the 5-day-old seedlings were transformed with Agrobacterium tumifaciens strain AGL cells comprising a binary vector with a selectable neomycin phosphotransferase II gene and a reporter gene encoding green fluorescent protein (GFP). Explants were cultured on a regeneration MS medium supplemented with ABA, and transformants were selected on the same medium (minus ABA and plus 70 mM AgNO3 and 15 mg/l kanamycin). The frequency of shoot regeneration on explant petioles was 30–40%. Transgenic plants were identified by GFP fluorescence and by polymerase chain reaction and Western blotting analysis. The transformation efficiency was as high as 75% of the total number of regenerated shoots. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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