| Popis: |
Fluorogenic reagents having a 2,1,3-benzoxadiazole (benzofurazan) moiety are reviewed in terms of reactivity, fluorescence characteristics, sensitivity and applicability in the biosciences. Those included are 4-fluro-7-nitro-benzofurazan (NBD-F), 4-( N,N -dimethylaminosulphonyl)-7-fluoro-2,1,3-benzoxadiazole (DBD-F), 4-aminosulphonyl-7-fluoro-2,1,3-benzoxadiazole (ABD-F), ammonium 7-fluoro-2,1,3-benzoxadiazole-4-sulphonate (SBD-F), 4-hydrazino-7-nitro-2,1,3-benzoxadiazole (NBD-H), 4-hydrazino-7-nitro-2,1,3-benzoxadiazole hydrazine (NBD-H NH 2 NH 2 ), 4-( N,N -dimethylaminosulphonyl)-7-hydrazino-2,1,3-benzoxadiazole (DBH-H), 4-aminosulphonyl-7-hydrazino-2,1,3-benzoxadiazole (DBD-PZ), (+)-4-nitro-7-(2-aminophyrrolidin-1-yl)-2,1,3-benzoxadiazole ( d -NBD-APy), (–)-4-nitro-7-(3-aminopyrrolidin-1-yl)-2-1,3-benzoxadizole ( l -NBD-APy), 4- N,N -dimethylamino-7 N -piperazino-2,1,3-benzoadiazole (DBD-PZ), 4- N,N -dimethylamino-7- N -cadaverino-2,1,3-benzoxadiazole (DBD-CD), 4-(aminosulphonyl)-7-(1-piperazynyl)-2,1,3-benzoxadiazole (ABD-PZ), 4-(aminosulphonyl)-7-(5-aminopentylamino)-2,1,3-benzoxadiazole (ABD-AP), 4-(aminosulphonyl)-7-(2-aminoethyl)-2,1,3-benzoxadiazole (ABD-AE) and ammonium 7- N -piperazino-2,1,3-benzoxadiazole-4-sulphonate (SBD-P2). Their application data in liquid chromatography are listed in tables. Future trends for the benzofurazan reagents are discussed. |
