| Autor: |
Julia Nikolaevna Khantakova, Vasily V. Kurilin, Vadim Kozlov, Natalya Kiryshina, Andrey Sokolov, Sergey Sennikov, Maria Kuznetsova, Julia Shevchenko, Julia A. Lopatnikova, Alexander Alexandrovich Khristin, S V Sidorov, Amir Maksyutov, Ekaterina Kulikova, I. A. Obleukhova, Alexander Vitsin |
| Rok vydání: |
2021 |
| Předmět: |
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| DOI: |
10.21203/rs.3.rs-696890/v1 |
| Popis: |
Dendritic cells (DCs) loaded with tumor-associated antigens (TAA) are known to be the important agents in antitumor response realization and still figure in lots of treatment schemes in cancer immunotherapy research. Here, we evaluated a cell-based protocol involving the use of original DNA constructs encoding the wide range of TAA epitopes expressed on different epithelial cancers. The constructs were transfected into ex-vivo-generated DCs of cancer patients (breast cancer, colorectal cancer, and non-small cell lung cancer). Direct cytotoxicity assay of effector cells, activated with the transfected DCs, showed a significant increase in the cytotoxicity against autologous tumor cells. The use of DNA-constructs encoding a large number of TAA’s for the in vitro DC loading to activate the T cell response could be a reliable and unified approach for immunotherapy and relapse prevention in patients with epithelial cancers. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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