Beijerinckia indica L3 fermentation for the effective production of heteropolysaccharide-7 using the dairy byproduct whey as medium

Autor:	Jin-Woo Lee, Sung-Koo Kim, Jeong Hwa Son, Ki Myong Kim, Jian-Rong Wu
Rok vydání:	2006
Předmět:	Beijerinckia indica biology Strain (chemistry) Chemistry Rhamnose Mutant Mutagenesis (molecular biology technique) Bioengineering Applied Microbiology and Biotechnology Biochemistry chemistry.chemical_compound biology.protein Fermentation Beta-galactosidase Food science Lactose
Zdroj:	Process Biochemistry. 41:289-292
ISSN:	1359-5113
DOI:	10.1016/j.procbio.2005.06.001
Popis:	A mutant strain of Beijerinckia indica L3 screened by a traditional chemical mutagenesis was grown in lactose-based mineral salts medium (MSM) for the production of heteropolysaccharide-7 (PS-7). The beta-galactosidase was expressed constitutively in the mutant and its activity was higher than that in parent strain. The highest amount of PS-7 produced by the mutant was 2.88 g/L with a viscosity of 4530 cP in lactose-based MSM medium. The PS-7 production was enhanced by the addition of 4 g/L glucose into lactose-based MSM medium, reaching 5.52 g/L with a viscosity of 39531 cP. PS-7 of 6.18 g/L with a viscosity of 45772 cP was produced from the mutant grown in whey medium. The PS-7 production from the mutant reached 7.04 g/L when 4 g/L glucose was added to the whey medium. Compositional analysis of PS-7 produced by the mutant grown in whey medium or lactose-based mineral salts medium showed that the ratios of glucose/rhamnose were maintained in the range similar to that of parent strain, indicating the same compositional structures of PS-7.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::cc3312c32960d38c8ca68b1e46c43116 https://doi.org/10.1016/j.procbio.2005.06.001 Zobrazit plný text záznamu Full Text from ScienceDirect