Increased ethanol production and tolerance by a pyruvate-negative mutant of Clostridium saccharolyticum

Autor:	K. B. Wemyss, W. D. Murray, A. W. Khan
Rok vydání:	1983
Předmět:	Ethanol Mutant Wild type General Medicine Acetate kinase activity Xylose Applied Microbiology and Biotechnology Microbiology Acetic acid chemistry.chemical_compound Biochemistry chemistry Ethanol fuel Kinase activity Biotechnology
Zdroj:	European Journal of Applied Microbiology and Biotechnology. 18:71-74
ISSN:	1432-0614 0171-1741
DOI:	10.1007/bf00500827
Popis:	To improve the conversion of hexoses and pentoses to ethanol, a pyruvate-negative (PN) mutant of Clostridium saccharolyticum, having lower acetate kinase activity, was obtained. The PN mutant used more substrate (glucose or xylose) and produced more biomass and ethanol, but less acetic acid. This shift in catabolism raised the ethanol/acetate ratio from 6.7 to 13. The PN mutant converted both glucose and xylose to ethanol at an efficiency of 80% of the theoretical yield as compared to 64% for C. saccharolyticum wild type. This improved production of ethanol was also accompanied by an increased tolerance to ethanol. The PN mutant showed 50% growth inhibition at an ethanol concentration of 6.5% (v/v) as compared to 3.5% for the parent strain.
Databáze:	OpenAIRE
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