Near Infrared Luminescent Oxygen Nanosensors with Nanoparticle Matrix Tailored Sensitivity

Autor:	Wenzhe Fan, Sang Man Koo, Christen Strollo, Raoul Kopelman, Yong Eun Koo Lee, Gwangseong Kim, Hoejin Hah, Elyse E. Ulbrich, Rajan Gurjar
Rok vydání:	2010
Předmět:	Matrix (chemical analysis) Polyacrylamide Hydrogel Nanosensor Chemistry In vivo Near-infrared spectroscopy chemistry.chemical_element Nanoparticle Nanotechnology Luminescence Oxygen Analytical Chemistry
Zdroj:	Analytical Chemistry. 82:8446-8455
ISSN:	1520-6882 0003-2700
DOI:	10.1021/ac1015358
Popis:	The development of sensors for noninvasive determination of oxygen levels in live cells and tissues is critical for the understanding of cellular functions, as well as for monitoring the status of disease, such as cancer, and for predicting the efficacy of therapy. We describe such nontoxic, targeted, and ratiometric 30 nm oxygen nanosensors made of polyacrylamide hydrogel, near-infrared (NIR) luminescent dyes, and surface-conjugated tumor-specific peptides. They enabled noninvasive real-time monitoring of oxygen levels in live cancer cells under normal and hypoxic conditions. The required sensitivity, brightness, selectivity, and stability were achieved by tailoring the interaction between the nanomatrix and indicator dyes. The developed nanosensors may become useful for in vivo oxygen measurements.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::cc29aeeaa2589ee97f1c4d4b87256262 https://doi.org/10.1021/ac1015358 Zobrazit plný text záznamu