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Genetic markers are indispensable for molecularand statistical genetic research involving nonhumanprimates. Genetic markers must be used to ascertainparentage and to confirm the accuracy of pedigreesbased solely on housing or demographic records;otherwise, the results of pedigree, linkage, orquantitative genetic analyses may be unreliable. Untilrecently, most genetic markers used in nonhuman primateswere plasma proteins or isozyme polymorphisms,which were required in large numbers, because levels ofgenetic variation revealed by these markers were ratherlow. We compared the newer, PCR-amplified shorttandem repeat markers (STRs) with a panel ofclassical biochemical polymorphic markers, for paternitydetermination among captive-bred rhesus monkeys. The STRmarkers exhibited an average genetic diversityof 64% and an expected paternity exclusionprobability of 0.443. Both of these were greater thanthe average 54.5% genetic diversity and 0.298 exclusionprobability exhibited by the biochemical markers.The STRs were much more efficient than thebiochemical markers for parentage determination, sincethey required only half the amount of genetic typingdata to resolve an average paternity case. Thus, theresults of applying these two classes ofgenetic markers in paternity tests were somewhatdifferent than expected on the basis of theoreticalexclusion probabilities. These differences were probablydue to inbreeding and other genetic differencesamong breeding colonies. Because they are moreinformative and provide rapid and efficient geneticdata, STRs are now the method of choice for parentagedetermination and pedigree corroboration among nonhumanprimates. |
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