PCR primers for microsatellite loci in a Madagascan waterbird, the Sakalava Rail (Amaurornis olivieri)

Autor:	Marc Rabenandrasana, Tamás Székely, Sama Zefania, Edward G. Brede, Peter R. Long, Michael William Bruford
Rok vydání:	2010
Předmět:	Canirallus Genetics biology Range (biology) Sakalava rail Canirallus kioloides Zoology Amaurornis biology.organism_classification Genetic marker Polymorphism (computer science) Microsatellite Ecology Evolution Behavior and Systematics
Zdroj:	Conservation Genetics Resources. 2:273-277
ISSN:	1877-7260 1877-7252
Popis:	We isolated 76 microsatellite loci from the Sakalava rail (Amaurornis olivieri) genome. These were tested in 30 individuals from six sites (in three regions) across the species’ range in Madagascar. Thirty-four of the 76 loci produced unambiguous polymorphic markers, with mean H e = 0.544 (range 0.0678–0.885), mean H o = 0.430 (range 0.0400–0.767) and mean N a = 4.59 (range 2–10). In addition, we tested all primers for amplification in the Wood rail, Canirallus kioloides (N = 3) and the Bemaraha rail, Canirallus sp. nov (N = 3). Twelve of the loci produced successful amplification with eight loci showing polymorphism.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::cc1b66e35a580b6018951143ebbfed34 https://doi.org/10.1007/s12686-010-9189-2 Zobrazit plný text záznamu Full text from SpringerLink