pH-Responsive Guest Binding of Polypeptide Containing a Cyclodextrin at the Terminal

Autor:	Toshihiro Aso, Yoshiharu Tsujita, Takatoshi Kinoshita, Yoshitomo Nagata, Hiroaki Yoshimizu, Norihiko Minoura
Rok vydání:	1994
Předmět:	chemistry.chemical_classification Circular dichroism Aqueous solution Cyclodextrin Stereochemistry Aqueous two-phase system General Chemistry Random coil Fluorescence spectroscopy Inclusion compound carbohydrates (lipids) chemistry.chemical_compound End-group chemistry polycyclic compounds
Zdroj:	Bulletin of the Chemical Society of Japan. 67:495-499
ISSN:	1348-0634 0009-2673
DOI:	10.1246/bcsj.67.495
Popis:	Poly(γ-methyl L-glutamate-co-L-glutamic acid) containing a β-cyclodextrin at the terminal (MG/GA-CyD) was prepared and the relationship investigated between the conformation of the polypeptide and inclusion capability of the β-cyclodextrin by circular dichroism and fluorescence spectroscopy in aqueous solution containing 8-anilino-1-naphthalenesulfonate (ANS) as a guest molecule. The terminal β-cyclodextrin of MG/GA-CyD could not include ANS at higher pH, where the polypeptide almost formed a random coil. This was attributed to the terminal β-cyclodextrin being concealed in the random coil of the polypeptide. At low pH, however, random coil-to-α-helix transition of the polypeptide exposed the terminal β-cyclodextrin to the aqueous phase resulting in the ANS-inclusion capability. These results imply that control of the polypeptide conformation leads to conformational switched guest binding of the terminal cyclodextrin.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::cc08a439ca56f5ce77803e779ec8425c https://doi.org/10.1246/bcsj.67.495 Zobrazit plný text záznamu