Enzyme-mediated free radical polymerization of acrylamide in deep eutectic solvents
| Autor: | Josué D. Mota-Morales, Gabriel Luna-Bárcenas, A. Reséndiz-Rubio, R. J. Sánchez-Leija, José Román Torres-Lubián |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
chemistry.chemical_classification
Aqueous solution General Chemical Engineering Polyacrylamide Radical polymerization 02 engineering and technology General Chemistry Polymer 010402 general chemistry 021001 nanoscience & nanotechnology Photochemistry 01 natural sciences 0104 chemical sciences Freezing point Solvent chemistry.chemical_compound chemistry Acrylamide Molar mass distribution Organic chemistry 0210 nano-technology |
| Zdroj: | RSC Advances. 6:13072-13079 |
| ISSN: | 2046-2069 |
| DOI: | 10.1039/c5ra27468k |
| Popis: | Deep eutectic solvents (DES) have attracted considerable attention as reaction media in biocatalysis due to their particular solvent properties. It has been demonstrated that some enzymes do not denature in these solvents and that under certain conditions, enzyme-catalyzed reactions in DES can result in higher conversions and reaction rates than those obtained in conventional media. In this work we report the enzyme-mediated free radical polymerization of acrylamide in nearly non-aqueous choline chloride–urea (CCl–U) and choline chloride–glycerol (CCl–Gly) DES. The catalytic activity of the enzyme, horseradish peroxidase (HRP), was observed to be lower at high DES concentrations than in phosphate buffer solution, whereas the thermal stability was enhanced. According to fluorescence and UV-vis spectroscopy studies, the drop in the enzyme activity could be a consequence of the partial denaturation of HRP in the “hydrated” DES. Despite this significant loss in activity, HRP was still able to initiate the free radical polymerization of acrylamide with full monomer conversions. For the CCl–U system, polymers with similar average molecular weight and slightly narrower polydispersities compared to those synthesized in a totally aqueous environment were obtained. Furthermore, taking advantage of the low freezing point of CCl–Gly, it was possible to synthesize polyacrylamide at 4 °C, while no polymer was obtained using water at the same temperature. These results illustrate the potential of DES to enable biotransformations in a wide range of temperatures, leading for instance to new strategies in materials synthesis, such as enzymatic cryo-polymerization. |
| Databáze: | OpenAIRE |
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