Overlapping Yet Distinct Promoter Activities of Two Arabidopsis Genes Coding for Nearly Identical Isoforms of the Acyl Carrier Protein

Autor:	Matthew G. Vander Heiden, Scott R. Baerson, Gayle K. Lamppa
Rok vydání:	1998
Předmět:	Gene isoform Reporter gene food.ingredient biology fungi Lateral root food and beverages Promoter Plant Science biology.organism_classification Molecular biology Acyl carrier protein food Arabidopsis Botany biology.protein Gene Ecology Evolution Behavior and Systematics Cotyledon
Zdroj:	International Journal of Plant Sciences. 159:533-538
ISSN:	1537-5315 1058-5893
DOI:	10.1086/297570
Popis:	Acyl carrier protein is encoded by at least five genes in Arabidopsis. Isoforms encoded by genes Acl1.2 and Acl1.3 differ by a single amino acid. We have examined the expression patterns directed by the Acl1.2 and Acl1.3 gene promoters in transgenic Arabidopsis using the reporter gene coding for s-glucuronidase (GUS). Fluorometric and histochemical analyses showed that the two promoters have overlapping yet distinct patterns of activity in both vegetative and reproductive tissues. In germinating seeds, the Acl1.2 promoter directed expression in all tissues at 2 days after imbibition (DAI) and then gradually became restricted to the elongation zone of the root apex, the root-hypocotyl transition zone, shoot apex, and cotyledon vasculature by 6 DAI. Acl1.3 promoter down-regulation was evident only in the basal hypocotyl region during this same time period. In young plants, Acl1.2 promoter activity was restricted to apical regions and within lateral root primordia. In anthesis-stage flowers, Acl1.2 was expre...
Databáze:	OpenAIRE
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