Interspecific hybridization inBrassica: Application of flow cytometry for analysis of ploidy and genome composition in hybrid plants

Autor:	J. Doležel, P. S. Sabharwal
Rok vydání:	1993
Předmět:	Genetics Brassica carinata Ovary (botany) Plant Science Horticulture Biology biology.organism_classification Genome Nuclear DNA chemistry.chemical_compound chemistry Botany Ploidy Ovule DNA Hybrid
Zdroj:	Biologia plantarum. 35:169-177
ISSN:	1573-8264 0006-3134
DOI:	10.1007/bf02925934
Popis:	Interspecific hybrids from the crosses betweenBrassica campestris, B. carinata, B. juncea andB. napus were obtained throughin vitro ovary and ovule culture. F1 hybrids were studied morphologically and flow cytometry was used to estimate 2C nuclear DNA content both in parentalBrassica species and their hybrids. It was found that in comparison with the A genome, the B and the C genomes ofBrassica contained 26.9 % and 43.9 % more DNA, respectively. This finding may be used to distinguish interspecific hybrids containing various genome combinations. It was concluded that flow cytometric analysis of nuclear DNA content might be useful tool inBrassica breeding.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::cbf4dcb42bac1d3a4b41410c0e5a5c9b https://doi.org/10.1007/bf02925934 Zobrazit plný text záznamu Full text from SpringerLink