Liquid Chromatographic Method for Simultaneous estimation of Thiocolchicoside and Etoricoxib from Tablet formulation

Autor:	Ravindra L. Bakal, Chaitanya A. Gulhane, Wrushali A. Panchale, Jagdish V. Manwar
Rok vydání:	2021
Předmět:	03 medical and health sciences 0302 clinical medicine Chromatography Thiocolchicoside Chemistry medicine 02 engineering and technology 021001 nanoscience & nanotechnology 0210 nano-technology Etoricoxib 030217 neurology & neurosurgery medicine.drug
Zdroj:	Asian Journal of Pharmaceutical Analysis. :118-122
ISSN:	2231-5675 2231-5667
DOI:	10.52711/2231-5675.2021.00020
Popis:	A new simple, reproducible and efficient liquid chromatographic method (RP-HPLC) was developed for simultaneous estimation of thiocolchicoside and etoricoxib for tablet formulation. Formulation containing thiocolchicoside and etoricoxib is used as analgesic. Separation was achieved by Nucleosil (4.6mm I.D × 250 mm) C18 column with mobile phase consist of Acetonitrile: water (0.05% Orthophosphoric acid V/V) in the ratio 25:75 at flow rate 0.7ml . The detection was carried out at 220nm. The retention time of thiocolchicoside and etoricoxib was found to be 3.75 min and 6.13 min respectively. Linearity of THC and ETR was found to be in the range of 2-10µg/mL and 30-150µg/mL. Percent recovery obtained for THC and ETR were 99.98% and 99.69% respectively. The method was validated as per ICH guidelines.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::cbd066b10172be1c0cf79d308d6a53f6 https://doi.org/10.52711/2231-5675.2021.00020 Zobrazit plný text záznamu