| Autor: |
Canying Hu, Lianyun Wu, Xianghong Ju, Ru-Min Jia, Minglong Ma, Biao Fang, Yanhong Yong, Tianyue Yu, Junyu Li |
| Rok vydání: |
2019 |
| Předmět: |
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| Zdroj: |
Frontiers in Molecular Immunology. 1:3-12 |
| ISSN: |
2630-5429 |
| Popis: |
Heat stress induces suppressor of cytokine signaling (SOCS) 1 and SOCS2 expression in the intestinal gut and disrupts inflammatory cytokine production in pigs. These changes may be important to the development of inflammatory bowel disease in heat-stressed pigs. However, the underlying mechanisms have not yet been completely elucidated. In the present study, we examined the roles of SOCS1 and SOCS2 in regulating the nuclear factor (NF)-κB pathway in CRL-2845 porcine macrophages. Ectopic expression of HSP70 significantly modulated NF-κB activity (p ≤ 0.05). Moreover, co-expression of SOCS1 or SOCS2 with HSP70 reduced NF-κB activity, which was abolished by SOCS1 or SOCS2 knockdown with small interfering RNA. Additionally, myeloid differentiation factor 88 (MyD88)-adaptor-like (Mal) protein was down-regulated in cells expressing SOCS1 and SOCS2. SOCS1 and SOCS2 were found to negatively regulate the activity of NF-κB induced by HSP70 overexpression by degrading Mal. These findings may facilitate the development of novel SOCS1-based and SOCS2-based therapeutic strategies for controlling heat stress-related disorders in pigs. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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