Hensen's node regulates avian neural crest differentiationin vitro
| Autor: | Gabrielle G. Leblanc, Timothy E. Holbert |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Neural fold
medicine.medical_specialty Cell type biology General Neuroscience Neural tube Neural crest Phenotype Cell biology Fibronectin Cellular and Molecular Neuroscience Endocrinology Cranial neural crest medicine.anatomical_structure Internal medicine medicine biology.protein sense organs Neural crest cell migration |
| Zdroj: | Journal of Neurobiology. 29:249-261 |
| ISSN: | 1097-4695 0022-3034 |
| DOI: | 10.1002/(sici)1097-4695(199602)29:2<249::aid-neu9>3.0.co;2-a |
| Popis: | Different anteroposterior (AP) regions of the neural crest normally produce different cell types, both in vivo and in vitro. AP differences in neural crest cell fates appear to be specified in part by mechanisms that act prior to neural crest cell migration. We, therefore, examined the possibility that the fates of neural crest cells, like those of neural tube cells, can be regulated by interactions with Hensen's node. Using a transfilter co-culture system, we found that young (stage 3+ to 4) Hensen's node up-regulates the expression of two cranial-specific phenotypes (fibronectin and smooth muscle actin immunoreactivities) in mass cultures of trunk neural crest cells, and down-regulates the expression of a trunk-specific phenotype (melanin synthesis). The changes in phenotype produced by exposure to young Hensen's node were not accompanied by changes in the proliferation of either fibronectin immunoreactive cells or melanocytes. The capacity of Hensen's node to elicit changes in trunk neural crest cell phenotype decreased as the developmental age of the node increased and was lost by stage 6. In addition, old Hensen's node did not stimulate the expression of trunk-specific phenotypes in cranial neural crest cells, suggesting that cranial- and trunk-specific phenotypes are induced by different mechanisms. |
| Databáze: | OpenAIRE |
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