| Popis: |
The availability of yeast lipid mutants is beginning to have an important impact in unravelling the relationship between mitochondrial membrane lipids and proteins. The use of sterol (Parks, 1978) and fatty acid auxotrophs (Lands and Graff, 1981, Trivedi et al., 1986) has provided information on several aspects of the structural-functional relationships that exist in membranes of varying lipid composition. Recently we have resorted to using Saccharomyces cerevisiae mutants which are defective in various enzymes responsible for the synthesis of phospholipids to probe these relationships further (Trivedi et al., 1987). These studies not only have emphasized the regulatory role of lipids, but have afforded an insight into their possible control of the assembly of mitochondrial membranes. Although we have been able to manipulate the phospholipid composition of yeast mitochondrial membranes significantly, the overall net charge of these membranes has been conserved due to biological compensation. |
