MITOCHONDRIAL MEMBRANE-BOUND STEROID HYDROXYLATING SYSTEMS: STRUCTURE AND FUNCTIONS

Autor:	Lapko Ag, Vadim L. Chashchin, A.A. Akhrem, Lapko Vn, Martsev Sp, Vladimir M. Shkumatov
Rok vydání:	1980
Předmět:	endocrine system Adrenal ferredoxin Cytochrome Adrenal cortex medicine.medical_treatment Biology Mitochondrion Adrenodoxin reductase Steroid medicine.anatomical_structure Biochemistry Adrenodoxin medicine biology.protein Inner mitochondrial membrane
DOI:	10.1016/b978-0-08-023967-5.50021-8
Popis:	A phenomenon of self-association has been shown for in vitro steroid hydroxylating system from adrenal cortex mitochondria. The data obtained testify to the presence of independent specific sites for binding with adrenodoxin reductase and cytochrome P-450 in the adrenodoxin molecule. Conditions for the reconstitution of the Sepharose-immobili-zed Fe2S2 adrenodoxin cluster have been found. The results of the limited trypsin digestion of the 20S, 22R-cholesterol hydroxylating cytochrome P-450 are presented. Proteolyzed cytochrome P-450 has been shown to retain its functional activity for a certain period of time both with a reconstituted in a solution 20S, 22R-cholesterol hydroxylating system and with self-association of the system on a column with immobilized adrenodoxin.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::cb90b24cb2ef6ccd56dbff867c733db7 https://doi.org/10.1016/b978-0-08-023967-5.50021-8 Zobrazit plný text záznamu