Micro Multiple Immunoassay Using Silicon-Based Microfabricated Protein Biochip

Autor:	Chisato Okamura, Yoshihiro Ashihara, Mitsuo Isomura, Takashi Shirakawa, Nobuyuki Ise
Rok vydání:	2002
Předmět:	Chromatography Materials science Silicon medicine.diagnostic_test Square Centimeter chemistry.chemical_element Chip Fluorescence Silicon based chemistry Immunoassay medicine Sandwich immunoassay Biochip
Zdroj:	Micro Total Analysis Systems 2002 ISBN: 9789401039529
DOI:	10.1007/978-94-010-0295-0_79
Popis:	We have developed multiple parallel immunoassay using silicon-based protein chip for parallel quantification of specific proteins. We employed micro fabricated chips with 10,000 pillars per square centimeter. The chip is composed of six lanes with 5x50 spotting sites. Several target proteins, i.e., alpha-fetoprotein (AFP), IL-6, MAPK and H-Ras were measured by fluorescent sandwich immunoassay. Positive signals were obtained simultaneously in each reaction site on the chip. Furthermore, IL-6 showed dose-dependent signals ranging with 5pg/mL to 50ng/mL.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::cb8e8210435c130f688986bd05b06e95 https://doi.org/10.1007/978-94-010-0295-0_79 Zobrazit plný text záznamu