Comparison of cytotoxicity of various surfactants tested on normal human fibroblast cultures using the neutral red test, MTT assay and LDH release

Autor:	P. Rivalland, Laurence Coiffard, C. Coiffard, Y. De Roeck-Holtzhauer, B. Arechabala
Rok vydání:	1999
Předmět:	chemistry.chemical_compound Neutral red Chromatography chemistry Pulmonary surfactant Benzethonium chloride Sodium Sorbitan monostearate Sorbitan monolaurate chemistry.chemical_element MTT assay Toxicology Cytotoxicity
Zdroj:	Journal of Applied Toxicology. 19:163-165
ISSN:	1099-1263 0260-437X
DOI:	10.1002/(sici)1099-1263(199905/06)19:3<163::aid-jat561>3.0.co;2-h
Popis:	We used the neutral red test, MTT assay and lactate dehydrogenase (LDH) release to compare the potential cytotoxicity of six surfactants belonging to different classes--three non-ionic surfactants (Triton x100, octylphenoxypolyethoxy alcohol, from Orion; Tween 60, polyoxyethylene (20) sorbitan monostearate, from ICI Speciality Chemicals; Tween 80, polyoxyethylene (20) sorbitan monolaurate, from Labosi), two anionic surfactants (Texapon K1298, sodium lauryl sulphate, from Henkel; Texapon N40, sodium laurylether sulphate, from Henkel) and one cationic surfactant (benzethonium chloride, from Siber Hegner)--on human fibroblast cultures. According to the LC50 (microg ml(-1)), the tested surfactants can be classified in the following order of increasing cytotoxicity: Tween 80 < Texapon N40 < Tween 60 < Texapon K1298 < Triton x100 < benzethonium chloride.
Databáze:	OpenAIRE
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