| Autor: |
Marwa El-Sayed, Asmaa Sayed Abdelgeliel, Aml Ghanem, Abdulmohsen H Alrohaimi, Seham Saeed Alzahrani, Eman Fayad, Mashail A Alghamdi, Ingy Badawy, Ahmed Bastawy, Dalia Elebeedy |
| Rok vydání: |
2023 |
| DOI: |
10.1101/2023.01.11.523569 |
| Popis: |
PurposeThere is an immense need to develop new antiviral compounds that are effective and have little side effects. Natural compounds provide a good candidate that is gaining popularity. Probiotics asLactiplantibacillus plantarumand plant-derived medicines as star anise extract have been investigated to treat viral diseases, cancer, and inflammation. This work aimed to investigate the antiviral effect of probiotics (Lactiplantibacillus plantarum) and (star anise) againstHerpes simplex virustype 1(HSV-1) andVesicular stomatitis virus(VSV).Patients and methodsLactiplantibacillus plantarumand star anise extract had been prepared and tested against vero cell lines for cytotoxicity assay. The antiviral effect ofL. plantarumand star anise against HSV-1 and VSV was evaluated by titration calculation (TCID50). The expression ofMXgenes were measured in infected cells before and after treatment withL. plantarumand star anise.ResultsWe found thatL. planterumis more effective againstHSV-1and caused 2.5 log reduction in virus titre while star anise extract is more effective against VSV and caused 1.25 log reduction in virus titre. Evaluation ofMXgenes expression revealed higher expression in HSV-1 infected cell treated withL. planterumand VSV infected cells treated with star anise extract.ConclusionL. planterumand star anise could be useful antiviral natural compounds that have minimal side effectsPlain language SummaryBothHerpes simplex virustype 1(HSV-1) andVesicular stomatitis virus(VSV) caused infection characterized by lytic lesions.Lactiplantibacillus plantarumand star anise had shown promising antiviral effects. So, we investigated the antiproliferative effect ofL. plantarumand star anise against HSV-1 and VSV by assessment of virus titre before and after treatment and assessment ofMXgene coding for antiviral proteins. We found thatL. plantarumand star anise could inhibit proliferation of HSV-1 and VSV and increase expression ofMXgene.L. plantarumand star anise could be good antiviral candidate. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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