Communications: Comparison of Fall and Spring Tumors as Inocula for Experimental Transmission of Walleye Dermal Sarcoma

Autor:	Gregory A. Wooster, Paul R. Bowser, Rufina N. Casey, James W. Casey, Sandra L. Quackenbush
Rok vydání:	1996
Předmět:	Epsilonretrovirus Pathology medicine.medical_specialty Inoculation Transmission (medicine) Aquatic Science Biology medicine.disease biology.organism_classification Aquatic organisms medicine Walleye dermal sarcoma virus Viral rna Sarcoma Northern blot
Zdroj:	Journal of Aquatic Animal Health. 8:78-81
ISSN:	1548-8667 0899-7659
DOI:	10.1577/1548-8667(1996)008<0078:ccofas>2.3.co;2
Popis:	To determine if the walleye dermal sarcoma virus (WDSV) was present in both spring regressing tumors and fall developing tumors, an experimental transmission trial was conducted with cell-free tumor filtrates. A relatively high portion (82%) of young-of-the-year (age-0) walleyes Stizostedion vitreum inoculated with filtrates of spring-collected tumors developed dermal sarcomas. Conversely, no dermal sarcomas were observed on age-0 walleyes inoculated with filtrates of a tumor collected in the fall. Northern blot analysis demonstrated high levels of WDSV RNA in spring tumors and in inoculum derived from spring tumors, but very little viral RNA was detectable from fall tumors or inoculum derived from fall tumors.
Databáze:	OpenAIRE
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