Improved separation of metallothionein isoforms by the presence of cyclodextrin in capillary zone electrophoresis

Autor:	Pål A. Olsvik, Bjørn Henrik Hansen, Valborg Holten, Rolf A. Andersen, Thore W. Wilhelmsen
Rok vydání:	2004
Předmět:	chemistry.chemical_classification Chromatography Cyclodextrin Organic Chemistry General Medicine Biochemistry Analytical Chemistry Inclusion compound Amino acid chemistry.chemical_compound Electrophoresis Isoelectric point Capillary electrophoresis chemistry Metalloprotein Metallothionein
Zdroj:	Journal of Chromatography A. 1051:237-245
ISSN:	0021-9673
DOI:	10.1016/s0021-9673(04)01389-5
Popis:	Capillary zone electrophoresis (CZE) with cyclodextrin (CD) in the polyacrylamide-coated capillary was used to study metallothionein (MT) forms in the horse kidney preparation produced commercially by Sigma. It is known that CDs form complexes with hydrophobic amino acids. The results show that the presence of CD improves the separability of the various MT forms, including the MT-IA and the MT-IB forms, metallothionein aggregates, as well as the so far unidentified a and b forms. This was true both below and above the isoelectric points (pIs), although the migration times were somewhat longer at increasing CD concentrations for runs at constant voltage than with constant current.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::cb64c9f0810da6354559c1d5bd916623 https://doi.org/10.1016/s0021-9673(04)01389-5 Zobrazit plný text záznamu Full Text from ScienceDirect