Detection of Radiation-Induced Hydrocarbons in Irradiated Fish and Prawns by Means of On-Line Coupled Liquid Chromatography−Gas Chromatography

Autor:	Georg A. Schreiber, Klaus Werner Bögl, Almuth Spiegelberg, Grit Schulzki
Rok vydání:	1997
Předmět:	chemistry.chemical_classification Degree of unsaturation Hydrocarbon Chromatography Chemistry General Chemistry Gas chromatography General Agricultural and Biological Sciences Mass spectrometry Halibut Alkadienes High-performance liquid chromatography Polyunsaturated fatty acid
Zdroj:	Journal of Agricultural and Food Chemistry. 45:3921-3927
ISSN:	1520-5118 0021-8561
DOI:	10.1021/jf970270+
Popis:	Radiation-induced hydrocarbons were analyzed in a fatty (halibut) and a lean fish (cod) as well as in a prawn species by on-line coupled liquid chromatography (LC)-gas chromatography (GC) combined with mass spectrometry. In irradiated halibut which is known to contain mainly saturated and monounsaturated fatty acids, all expected radiolytic alkanes, alkenes, and alkadienes could be detected. The yields of the C n-1 and C n-2:1 hydrocarbons were comparable to those found in irradiated lipids of terrestrial animals and plants. However, in cod and prawns which contain high levels of polyunsaturated fatty acids (PUFA), the C n-1 hydrocarbons were found in concentrations which were up to 10 times higher whereas the C n-2:1 products were again comparable to those of terrestrial animals and plants. The identification of radiation-induced hydrocarbons in fish lipids was achieved by transfer of the hydrocarbons from the LC column to the gas chromatographic column in fractions differing in their degree of unsaturation. For the first time, radiation-induced hydrocarbons with more than four double bonds generated from polyunsaturated fatty acids (20:4ω6 and 20:5ω3) could be identified.
Databáze:	OpenAIRE
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