| Autor: |
Klara Weipoltshammer, Sarah Stummer, Katrin Friederike Leesch, Andreas Beyer, Fabian Titz-Teixeira, Andrea Pauli, Anastasia Chugunova, Michelle Huth, Christian Schoefer, Laurenz Holcik, Andreas Lackner, Elena Galimberti, Julia Ramesmayer, Stefan L. Ameres, Merrit Romeike, Martin Leeb, Veronika A. Herzog, Marius Garmhausen, Laura Santini, Robert Sehlke, Christa Bücker, Arndt von Haeseler, Austin Smith |
| Rok vydání: |
2020 |
| Předmět: |
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| Popis: |
Cell fate transitions depend on balanced rewiring of transcription and translation programmes to mediate ordered developmental progression. Components of the nonsense-mediated mRNA decay (NMD) pathway have been implicated in regulating embryonic stem cell (ESC) differentiation, but the exact mechanism is unclear. Here we show that NMD controls the translation initiation factorEif4a2and its premature termination codon encoding isoform (Eif4a2PTC). NMD deficiency leads to translation of a specific truncated Eif4a2 protein, which elicits increased translation rates and causes significant delays in mouse ESC differentiation. Thereby a previously unknown feedback loop between NMD and translation initiation is established. Our results illustrate a clear hierarchy between KOs in severity of target deregulation and differentiation phenotype (Smg5>Smg6>Smg7), which highlights heterodimer-independent functions for Smg5 and Smg7. Together, our findings expose an intricate link between mRNA stability and translation initiation control that must be maintained for normal dynamics of cell state transitions. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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