Honorable Mention — Student Research Award 1995: Signal Transduction Mechanisms in Substance P‐Mediated Ciliostimulation
| Autor: | Thomas V. McCaffrey, Rodney J. Schlosser, Judith M. Czaja |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty business.industry Cilium Neuropeptide Substance P Nasal glands 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology 0302 clinical medicine medicine.anatomical_structure Endocrinology Otorhinolaryngology Mechanism of action chemistry Internal medicine Mole medicine Surgery medicine.symptom Respiratory system 030223 otorhinolaryngology business Respiratory tract |
| Zdroj: | Otolaryngology–Head and Neck Surgery. 113:582-588 |
| ISSN: | 1097-6817 0194-5998 |
| DOI: | 10.1177/019459989511300509 |
| Popis: | Substance P is a neuropeptide released by afferent neurons in the respiratory tract during inflammatory reactions. It produces effects on blood vessels, bronchial smooth muscle, nasal glands, and respiratory cilia. We studied the in vitro effect of substance P on the ciliary beat frequency of human adenoid explants and its mechanism of action. Substance P was added to cultured adenoid at concentrations of 10−10, 10−8, 10−6, and 10−4 mol/L. Ciliary beat frequency was determined with phase-contrast microscopy and microphotometry. Substance P increased ciliary beat frequency a maximum of 11.9% ± 3.8% (p < 0.01). Diclofenac (10−6 mol/L) significantly blocked the ciliostimulatory effects of SP (p < 0.022), indicating that prostaglandin synthesis is an intermediate step in the action of substance P on ciliary beat frequency. The L-arginine analogs, NG-nitro-L-arginine methyl ester and NG-monomethyl-L-arginine, inhibit nitric oxide synthesis from L-arginine. L-Arginine analogs (10−4 to 10−2 mol/L) inhibited the ... |
| Databáze: | OpenAIRE |
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