| Autor: |
Yong-Qing Hao, Shengbo Zheng, Rui Xing, Xuehui Cai, Wei-Jun Yang, Congli Yuan, Daoliang Lan, Zhibiao Yang, Li Cui, Xiuguo Hua, Jiangping Gu |
| Rok vydání: |
2010 |
| Předmět: |
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| Zdroj: |
2010 4th International Conference on Bioinformatics and Biomedical Engineering. |
| ISSN: |
2151-7614 |
| DOI: |
10.1109/icbbe.2010.5515736 |
| Popis: |
This study aimed to study the immune activity of glutamate dehydrogenase (GDH) of streptococcus suis serotype 2 and detect its activity. METHODS: The GDH gene was cloned and sequenced. Recombinant GDH protein was expressed in a prokaryotic expression system and purified by affinity chromatography. Immunocompetenc of the resulting protein was analyzed by western blotting with anti-GDH monoclonal antibody. RESULTS: The fusion protein was efficiently expressed. Western blotting analysis confirmed that the resulting protein was immunogenic. CONCLUSIONS: The GDH gene of S.suis 2 was successfully cloned and the high expressed, which facilitated the further studies on the development of immunological detection and vaccine design. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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