Effect of resveratrol on the in vitro maturation of ovine (Ovis aries) oocytes and the subsequent development of handmade cloned embryos
| Autor: | María del Carmen Navarro-Maldonado, Raymundo Rangel-Santos, Demetrio Alonso Ambriz-García, José Luis Martínez-Ibarra, Eugenia Adriana Espinoza-Mendoza |
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| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine chemistry.chemical_classification Reactive oxygen species biology Embryogenesis Embryo Blastomere Resveratrol biology.organism_classification 01 natural sciences In vitro maturation Andrology 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology chemistry embryonic structures Ovis Embryo quality 010606 plant biology & botany |
| Zdroj: | Veterinaria México OA. 5 |
| ISSN: | 2448-6760 |
| DOI: | 10.22201/fmvz.24486760e.2018.4.491 |
| Popis: | Veterinaria Mexico OA ISSN: 2448-6760 Cite this as: Martinez-Ibarra JL, Espinoza-Mendoza EA, Rangel-Santos R, Ambriz-Garcia DA, Navarro-Maldonado MDC. Effect of resveratrol on the in vitro maturation of ovine ( Ovis aries ) oocytes and the subsequent development of handmade cloned embryos. Veterinaria Mexico OA. 2018;5(4). doi: 10.22201/fmvz.24486760e.2018.4.491 The effect of resveratrol on the in vitro maturation (IVM) of ovine ( Ovis aries ) oocytes and the development of handmade cloned embryos was evaluated. The nuclear maturation and reactive oxygen species (ROS) levels in the oocytes, as well as the early development and morphological cloned embryo quality, were evaluated under different resveratrol concentrations (0, 0.5, 2 and 5 μM). After IVM, no significant difference was observed in the maturation rate of oocytes treated with 0.5 μM (81.3 %) and 2 μM (72 %) resveratrol compared to that of the control group (0 μM) (74.2 %), but the rate significantly decreased at 5 μM (56 %) (p 0.05). For cloned embryo development, the embryos obtained from the oocytes treated with 0.5 μM resveratrol showed higher (p |
| Databáze: | OpenAIRE |
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